|
Wk |
Date |
Topics |
Manual |
Text |
|
1 |
8/28 |
Introduction,
Lab procedures, Safety |
|
Handouts |
|
8/30 |
Microscopy |
Ex. 1, 5 |
|
|
|
2 |
9/4 |
Cyanobacteria,
Eukaryotic microbes |
Ex. 71-74 |
137-156 |
|
9/6 |
Microbiological
media, autoclaving |
Ex. 14, App. J |
62-70, 337 |
|
|
3 |
9/11 |
Hanging
drop slides, smear prep, staining, isolation of microbes |
Ex. 6, 8, 10, 11, 12. pg. 50 |
81-84, 97 |
|
9/13 |
Gram
staining, Environmental microbes |
Ex. 9 |
handout |
|
|
4 |
9/18 |
Streak,
spread and pour plates |
Ex. 16-18 |
62-63 |
|
9/20 |
Day 2 -
plates, Counting, Winogradsky column |
Ex. 21 |
handout |
|
|
5 |
9/25 |
Environmental
effects on microbes |
Ex. 20, 42-44 |
204-208 |
|
9/27 |
Day 2 -
Env. effects, handwashing |
Ex. 49 |
|
|
|
6 |
10/2 |
Disinfectants
and antimicrobial agents
(Nobel conference) |
Ex. 47-48 |
330-331, 345-350 |
|
10/4 |
Day
2- disinfectants |
|
|
|
|
7 |
10/9 |
MPN test
of sewage treatment plant water |
Ex. 51 |
848-851 |
|
10/11 |
Day 2 -
MPN, Enterotube II |
Ex. 39 |
|
|
|
8 |
10/16 |
Day 3 -
MPN, Day 2 - Enterotube II |
|
|
|
10/18 |
NO CLASS |
|
|
|
|
9 |
10/23 |
Bacterial
transformation |
Handout |
288-289 |
|
10/25 |
Day 2 –
transformation |
|
|
|
|
10 |
10/30 |
Streptococci
and isolation of normal body microbes |
Ex. 65, 66 & 68 |
570-581 393-398 |
|
11/1 |
Day 2 -
body microbes |
|
|
|
|
11 |
11/6 |
Bacterial
count of food products, yogurt production |
Ex. 54, 56 |
863-639 |
|
11/8 |
Day 2 -
bacterial count and yogurt |
|
|
|
|
12 |
11/13 |
Student experiments – Day 1 |
|
|
|
11/15 |
Student experiments – Day 2 |
|
|
|
|
13 |
11/20 |
Field
Trip |
|
|
|
11/22 |
NO CLASS |
|
|
|
|
14 |
11/27 |
Unknowns
- Day 1 |
|
|
|
11/29 |
Unknowns
- Day 2 |
|
|
|
|
15 |
12/4 |
Unknowns
- Day 3 |
|
|
|
12/6 |
ELISA |
handout |
510-511 |
|
|
16 |
12/11 |
Poster
presentations |
|
|
|
12/13 |
Poster
presentations |
|
|
Grading:
For weeks 1-12, there will be a 15-point grading opportunity each week.
Grading opportunities will include quizzes, handouts and formal lab
reports. You will be allowed to
drop the lowest grade of the 15-point lab grades.
The successful identification of your lab unknown is worth 20 points and
your poster will be worth 20 points. You
will also be required to maintain a lab notebook throughout the semester.
A handout further discusses this notebook.
Therefore the total possible points for lab is:
11 15-point lab grades = 165 pts.
Unknown identification =
20 pts.
Poster presentation = 20
pts.
Lab notebook
30 pts.
Total
235 pts.
Gross and minor infractions of lab safety and
equipment maintenance (e.g., microscope care) will result in point deductions.
Attendance:
Attendance is mandatory and no lab can be made up if missed.
Be prompt coming to lab as important announcements may be made at the
very beginning of lab. You are
responsible for any material or announcements you may have missed if you are
absent or tardy.
TIPS FOR
STAYING CAUGHT UP IN MICROBIOLOGY LAB:
1.
Stay calm, don't panic.
Labs may be rushed at first, but time will always be allotted the next
lab period to finish what you don’t finish in one lab period.
2.
Read the syllabus before each lab
period to know what you are doing that day.
Read the exercise in the lab manual before lab.
3.
As you read through the exercises before lab, highlight the PURPOSE and
EXPECTED RESULTS. Lab time is VERY
limited. You won’t finish if you have to waste time reading through the
experimental procedures.
4. Remember the organisms you are
working with are not all genetically identical, even if they are within the same
species classification. They may
not do what you expect them to do. Microbiology
is not a black and white science.
5. All assignments are due AT
THE BEGINNING of the lab period they are due.
Quizzes will be conducted at the beginning of each lab period or end of
lecture. Be ready to go at the beginning of each lab period.
6.
Always ask if uncertain.